Although inositol triphosphate (IP3) diffuses into the cytosol, diacylglycerol (DAG) stays close to the plasma membrane, due to its hydrophobic properties. Enzyme extracted in 1 M salt from membranes of rat brain homogenates made in the presence of phospholipase C was purified further by affinity chromatography on a column containing phosphatidylserine, diacylglycerol, and cholesterol immobilized in polyacrylamide. The restriction enzyme recognition sequences on both sides of the S gene are used. The gene transfer method is the Agrobacterium method, A commonly used method such as a particle gun method or an electroporation method is used. Diacylglycerol can be mimicked by phorbol esters. JSTOR®, the JSTOR logo, JPASS®, Artstor®, Reveal Digital™ and ITHAKA® are registered trademarks of ITHAKA. Can not. Therefore, there are problems in complexity of the production process and cost. FIG. INDUSTRIAL APPLICABILITY According to the present invention, by using a plant in which the function of the gene encoding diacylglycerol acyltransferase is inactivated, the desired diacylglycerol can be obtained immediately after oil extraction, and conventional industrial production Therefore, complicated synthesis steps and by-product removal steps that could not be avoided will be unnecessary, and it will be possible to supply diacylglycerol more inexpensively and stably. 1. As a method therefor, (1) a method of utilizing a transesterification reaction between fat and oil and glycerol , (2) there is a method using an esterification reaction using a fatty acid and glycerol, which is carried out using either a chemical reaction or an enzymatic reaction using an alkali (earth) metal hydroxide catalyst ( Japanese Unexamined Patent Publication No.                       HA20, Free format text: Diacylglycerol (DG) lipase is a key enzyme in the pathway of 2-arachidonoylglycerol (2-AG) biosynthesis (). Introduction of antisense gene into soybean cells (1) Coating the prepared vector with gold particles, It is introduced into the cultured cells of the plant using a particle gun. Genes that control the metabolism of the fitate in plants and uses of the same. 1 is an illustration of biosynthesis of triacylglycerol. All of these reactions require (1) a step of separating and purifying a desired product and a by-product, and (2) a purified fatty acid as a material. The present invention provides a method for producing diacylglycerol capable of performing the above, and a gene sequence that inactivates the function of the gene encoding diacylglycerol acyltransferase. that diacylglycerol is produced as a result of the hydrolysis of phosphatidylinositols by phospholipase C (1-3). In addition to activating PKC, diacylglycerol has a number of other functions in the cell: Synthesis of diacylglycerol begins with glycerol-3-phosphate, which is derived primarily from dihydroxyacetone phosphate, a product of glycolysis (usually in the cytoplasm of liver or adipose tissue cells). 1. In addition, cells (individuals) into which the gene has been introduced There is no particular limitation on the marker for selecting Escherichia coli, such as various antibiotics, herbicides, and plant hormone synthesis genes. This item is part of JSTOR collection important contributions to research and of work that appears to a Member to The detection of the target gene by mutation is carried out by DN because the mutation is a mutation generated in DNA. Gene recombination can be performed by a known gene recombination technique by creating a vector and introducing a gene. Find out how LUMITOS supports you with online marketing. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. Diacylglycerol is a precursor to triacylglycerol (triglyceride), which is formed in the addition of a third fatty acid to the diacylglycerol under the catalysis of diglyceride acyltransferase. 2001-64671, See W099 / 48378). Learn about this topic in these articles: lipid formation. Further, analysis of the expression pattern by Northern blot analysis revealed that strong expression was observed in immature seeds having a diameter of about 7 to 9 mm and that expression was induced by ethylene treatment. This observation formed the basis for the first step in a two-step purification of diacylglycerol kinase. In order to suppress the expression of the gene encoding diacylglycerol acyltransferase having the function of producing triacylglycerol from diacylglycerol in seeds, in the case of soybean, the diacylglycerol having the nucleotide sequence according to claim 2 is used. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE. Glycerol-3-phosphate is first acylated with acyl-coenzyme A (acyl-CoA) to form lysophosphatidic acid, which is then acylated with another molecule of acyl-CoA to yield phosphatidic acid. In order to induce the mutation, a treatment that damages the DNA is performed. A plasmid is prepared (the promoter used at this time is preferably a seed-specific expression promoter, but a constitutive promoter such as CaMV35S promoter or actin promoter may be used). As a result, the soybean diacylglycerol acyltransferase gene encodes a polypeptide consisting of 500 amino acids, and shows 59.9% and 62.0% homology at the amino acid level with Arabidopsis thaliana and tobacco diacylglycerol acyltransferase. Continued front page Reconstitution experiments showed that the soluble step 2 enzyme bound to inside-out vesicles of human erythrocytes only in the presence of diacylglycerol or phospholipase C but not phospholipase A2 or D. Redistribution of the kinase from soluble to vesicle-bound forms occurred rapidly and was dependent on the concentration of phospholipase C used to treat the vesicles.

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