Molecular detection of human H7N9 influenza A virus causing outbreaks in China, Influence of pressure changes during embryo transfer on blastocyst viability, Protective effects of HBSP on ischemia reperfusion and cyclosporine a induced renal injury, Alkaloid fraction of jarong (Achyranthes aspera Linn) leaf induced apoptosis breast cancer cell through p53 pathways, Analisis estructural e inmunohistoquimico de la atresia folicular de Vanellus chilenis (Charadriidae) e Himantopus melanurus (Recurvirostridae). This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us [email protected] While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. [3] Dauwerse et al. Thymine and cytosine, the bases which feature in the purines, both have a single ring structure.

- Primer Extension with Klenow exo- [4]

Shelf Life: 12 months after date of delivery, Molecular Formula: C43H65N4O21P3 (free acid), Molecular Weight: 1066.91 g/mol (free acid), Form: sterile clear aqueous solution in 10 mM Tris-HCl, Spectroscopic Properties: λmax 290 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl pH 7.5), Applications:Incorporation into DNA/cDNA by TUNEL (Terminal deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling) has been done by mammary tissue were sliced, then drooped with 50ul of TUNNEL labeling mix (consisting of 5ulenzymeterminal deoxynucleotydil transferase and 45ulfluorescein-dUTP), incubated at 37 [degrees]C for 30 minutes in the moist chamber. Can be diluted using sterile distilled water, preferably Milli-Q® water or can be diluted using sterile TE [10 mM Tris-HCl, 1 mM EDTA (pH 7.5)]. Your Basket/Online QuoteItems: 0 (0,00 €)

Our new RUO kit, the, Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. dUTP: 2'-Deoxyuridine 5'-Triphosphate: dUTP: Dhaka Urban Transport Project (World Bank; Bangladesh) email us, or call 1-800-632-7799. All Rights Reserved. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-based in situ Cell Death Detection Kit, Fluorescein (Roche, Nutley, NJ, USA) was used to detect the apoptosis of the photoreceptor cells in the sections. » Search & Order - Reverse Transcription with MMLV Reverse Transcriptase [6]

(2008) Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]. https://acronyms.thefreedictionary.com/dUTP, The terminal deoxynucleotidyl transferase, Apoptosis was evaluated by using terminal deoxynucleotydil transferase, (a-c) Values from three replicates with different superscripts denote a significant difference relative to other groups (p<0,05), TUNEL, terminal deoxynucleotidyl transferase, Keywords: Behcet disease, neuro-Behcet disease, immunoglobulin G, terminal deoxynucleotidyl transferase, H&E: hematoxylin and eosin; TUNEL: terminal deoxynucleotidyl transferase-mediated, Abbreviations: CMC, Carboxymethyl cellulose; CS, calf serum; DMSO, dimethyl sulfoxide; GC, gastric cancer; IC50, half maximal inhibitory concentration; MTP, mitochondrial transmembrane potential; MTT, 3-(4, 5-dimethylthiazol-2-yl)2, 5-diphenyltetrazolium bromide; PepE, peperomin E; PI, propidium iodide; PVDF, polyvinylidene fluoride; SC79, 6-chloro-a-cyano-3-(ethoxycarbonyl)-4H-1-benzopyran-4-acetic acid ethyl ester; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; TBST, Tris-buffered saline containing Tween 20; TUNEL, terminal deoxynudeotidyl transferase, Fortunately, most DNA polymerases, when presented with, En el contexto de pesquerias artesanales, uno de estos sistemas incluye la creacion y asignacion de Derechos de Uso Territorial Pesqueros (, For hematoxylin and eosin (H&E) staining and terminal deoxynucleotidyl transferase, Terminal deoxynucleotidyl transferase-mediated, The assay relies on the ability of the enzyme terminal deoxynucleotidyl transferase (TdT) to catalyze the addition of, Dictionary, Encyclopedia and Thesaurus - The Free Dictionary, the webmaster's page for free fun content, Effect of Phosphorylated-Extracellular Regulated Kinase 1/2 Inhibitor on Retina from Light-induced Photoreceptor Degeneration, The Effects of Monomers Used in Polymeric Biomaterials on Renal Tissue/ Efectos de los Monomeros de los Biomateriales Polimericos Utilizados en Tejido Renal, Native plants (Phellodendron amurense and Humulus japonicus) extracts act as antioxidants to support developmental competence of bovine blastocysts, Impact of neuro-Behcet disease immunoglobulin G on neuronal apoptosis, NRF2 Plays a Critical Role in Both Self and EGCG Protection against Diabetic Testicular Damage, The natural secolignan peperomin E induces apoptosis of human gastric carcinoma cells via the mitochondrial and PI3K/akt signaling pathways in vitro and in vivo, The elephant in the room: contamination control in molecular testing, Extraccion de recursos naturales en contextos de abundancia y escasez: un analisis experimental sobre infracciones a cuotas en areas de manejo y explotacion de recursos bentonicos en el centro-sur de Chile, White light-emitting diodes (LEDs) at domestic lighting levels and retinal injury in a rat model, Potential protection of Coeloglossum viride var. Please note: The optimal final concentration of Digoxigenin-11-dUTP may very depending on the application and assay conditions. Before commencing each PCR, fully assembled reactions are treated with UDG, which cleaves the uracil base in dUTP -containing DNA (amplicon) but does not react with dUTP and has no effect on authentic DNA template containing thymidine bases or RNA. Abbreviation for deoxyuridine 5-triphosphate. All content on this website, including dictionary, thesaurus, literature, geography, and other reference data is for informational purposes only. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Incorporation into RNA by Click publication title for the full text.

Contact your local subsidiary or distributor. dTTP, (deoxythymidine 5'-triphosphate), and dCTP, (deoxycytidine 5'-triphosphate), make up the pyrimidines. al (1991) Detection of Shiga Toxin-Producing Shigella dysenteriae Type 1 and Escherichia coli by Using Polymerase Chain Reaction with Incorporation of Digoxigenin-11-dUTP. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. Please enter a quantity for at least one size, DNA Modifying Enzymes & Cloning Technologies, DNA Assembly, Cloning and Mutagenesis Kits, Protein Expression & Purification Technologies, SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit, Thermostable Ligase Reaction Temperature Calculator, PCR, qPCR & Amplification Technologies Products. Selected References: This includes personalizing content and advertising.

Description: Digoxigenin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. Related products: Digoxigenin NT Labeling Kit, #PP-310-DIGX. J Clin Microbiol. [5] Schmitz et al. (2005) Incorporation of reporter-labeled nucleotides by DNA polymerases. To save your cart and view previous orders, sign in to your NEB account. Contains 0.25 ml of 100 mM ultrapure dUTP. » Sign in / Register, Digoxigenin-X-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt. Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.



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